The Mammalian Metabolism of L-Histidine
نویسندگان
چکیده
The major pathway of histidine metabolism in animals and bacteria other than incorporation into protein involves first deamination to urocanic acid, then the anaerobic addition of water to form an intermediate generally considered to be 4(5)imidazolone-5(4)-propionic acid (1, 2). Finally, the ring is split enzymatically to ~formiminoglutamic acid (3) which is converted to free cglutamic acid by transfer of the formimino group to tetrahydrofolic acid (4, 5). In the course of a study of the urinary metabolites of n-histidine-Cl4 in the rat and monkey, a new metabolite of the urocanic acid pathway was found and identified as hydantoin&propionic acid (6). In investigating the sequence of reactions between urocanic acid and hydantoin propionic acid, techniques were developed for stabilization and isolation of the intermediate, imidazolone propionic acid (7). This paper describes the enzymatic and nonenzymatic reactions of purified imidazolone propionic acid-2-Cl4 and demonstrates the direct enzymatic formation of L-hydantoin&propionic acid from this intermediate by an oxidase from the soluble fraction of guinea pig liver.
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